I am working on grass fungal endophytes bioprospecting, I have isolated the sec. metabolites by using Ethyl acetate and Methanol for culture filtrate and mycelial mat but I am having a small doubt that why can't we isolate fungal metabolites by sequential isolation by using various solvents. If yes, then which are the solvents used for it.
Here i am giving you the general idea of extraction procedure. You can use polar and non polar solvents for extraction first you should identify the metabolites and categorize it if its previously know and see the component and classify them for understanding and chose the solvent for dissolution ?
Regards
Dear Nischitha,
If I well understood your question, yes it is possible performing a successive extraction of fungal secondary metabolites, using different solvents according to their polarity. I propose these solvents: hexane, ethyl acetate, chloroform and methanol. However, ethyl acetate is recommended and rich with various interesting metabolites.
Good luck and best regards
Afra
I am agree with Rohit. Please find out the attached document. A fascinating article reported the significance of sold media on production of interesting secondary metabolites (SMs). they investigated the impact of different media types, liquid media and solid media, on the SMs production.
Hi,
You can use GC-MS method.
Please follow the added paper, I think it can be helpful to you.
Good luck
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts