After isolating T cell gDNA using DNAzol and resuspending in NaOH and HEPES, I quantified DNA recovery using a Qubit and my yield was only about 50%. I think I overdried the pellet and the DNA might not have fully resuspended. I am wondering how to improve resuspension, and if I can incubate the DNA at 37 degrees C (or warmer?) even though I have already frozen it at -20 overnight.
Prof. Gheita
Dear Max Lee
Please check this previous RG Q:
https://www.researchgate.net/post/Any_tips_for_resuspending_clumps_of_DNA
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Marwan E Majzoub
you can incubate it at 60-70oC for about 10 mins and this should help with resuspending the DNA
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Ammar Abdalrahem
To resuspend your DNA, keeping it at 37 degree water bath for 1-2 hrs.
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