22 November 2018 4 8K Report

I'm currently in the process of perfecting a protocol for the isolation of RNA from single zebrafish embryos at the 8ss to be used in RNA-sequencing.

I routinely get pretty poor 260/230 ratios (around 0.5) but decent 260/280 (1.9). I sent my samples to the bioanalyzer to have their RNA integrity number checked and have gotten values of 10 back repeatedly - for the sequencing platform I plan to use I need an RIN of >8.

So my question is can I just ignore the 260/230 ratio from the nanodrop given the RIN is 10.

Thanks

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