I am extracting DNA from Gastropod tissue using the E.Z.N.A Mollusc DNA kit. However, I am waiting for the kit to arrive and I was wiondering if anyone knows Can I homogenise samples using Liquid Nitrogen and then store them (7 days max) before adding them to the ML1 buffer and incubating?
If this does not affect the quality of genomic DNA then it will save me alot of time.
I have attached the EZNA Mollusc kit protocol
I think you could store your tissue in liquid nitrogen for rapid freezing then transfer it into -80℃ or just keep tissue in liquid nitrogen before you extract, it is okay to store in liquid nitrogen in short time.
I am not sure about the quality of homogenise samples which are pretrement. I always grind tissue then I extract DNA and my tissue samples are stored at -80℃ all the time (I am extracting genomic DNA of Echinoderms).
I hope it will help you.
Good luck for genomic DNA extraction！
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