04 April 2019 1 8K Report

I have 195 gastropod (Buccinum undatum) samples that have been extracted using the phenol-chloroform method and the same 195 extracted using the ENZA Mollusc DNA extraction Kit. Individually, I have had varying success with the methods. However, when combined I can choose 195 good High Molecular weight extractions that are suitable.

I will be doing library preperation using 195 samples for ddRAD (ApeKI and BamHI) and I am wondering if it is alright to use DNA extracted via different methods to construct a single library?

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