I am working on cancer stem like cells, when I re-plated the cancer cells on collagen type I coated plate in serum free medium, after 12 days I observed adherent rounded shape cell aggregates (sphere like) with about 300µm diameter. I want to know they are real spheroids? I mean, is it possible to obtain spheroids in adherent culture on collagen type I coated plates?
I am wondering if they are real spheroids! As I found in the literature people used ultra-low attachment plates (non adherent culture) or BD Matrigel in presence of serum free medium as a standard protocol for sphere formation. Could every one help me understand the mechanism underlying this protocols?
Dear Ms. Tizabi,
Thank you for your valuable information and your paper, also you can find one more reference in the literature (1). It seems they used plates coated with 100 µm collagen spots in which the regions excluding the collagen spots coated with ultra-low attachment materials. As I found no one used plates that completely were coated with collagen for sphere formation. However, Kirkland (2) used aformentioned plates in serum free medium, but he named them “colony” instead of sphere!!
1. Tamura T. et al, 2008.
2. Kirkland SC. 2009.
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