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18 January 2021 10,005 3 View

How do I resolve the following error I get during the execution of WRF gen_be for creating background error covariance file?

I am trying to generate the be.dat file using the gen_be facility of WRF. I have 12 hour and 24 hour outputs from WRF at 00 UTC and 12 UTC for a month. When I try to run gen_be_wrapper.ksh on my...

15 January 2020 244 2 View

Protein extraction from a non-cellular structure?

I am trying to look at protein in a non-cellular structure using SDS-PAGE. I will be collecting plenty of those structures from the model organism on different days. Is it wise if I collect...

12 January 2020 2,918 6 View

What happens to palladium [pd (0)] at the electrode surface during electro chemical sensing. Has it change its oxidation state?

What happens to palladium [pd (0)] at the electrode surface during electro chemical sensing. Has it change its oxidation state?

25 July 2019 2,600 1 View

From a mechanical engineering point of view, What are the functional requirements that are required in the design of the mid-sole of a shoe?

I am working on a project on designing and testing new materials and different structure(3D-infill) configurations as prototypes for the mid-sole of a running shoe. I was wondering about the...

02 April 2019 1,040 2 View

How to find structurally similar protein?

I have a protein obtained through ab initio modeling. I would like to know whether this protein is exhibiting any kind of structural similarities with other proteins available in the PDB. How I...

01 October 2017 7,058 4 View

Mice footpad histology protocol?

13 July 2017 5,533 5 View

Can any one suggest me how to compare the active sites of two different proteins?

I am trying to design multi-target directed ligands. I have found some similar common ligands for both proteins. When I docked these ligands I have seen the score is almost similar. But these...

08 March 2017 1,898 2 View

B factor comparison?

18 December 2016 3,013 3 View

What is the best protocol to check the expression of CD107a (LAMP-1) by Flow cytometry?

I need to titrate mouse CD107a antibody to be used for my future CD8+ T cell degranulation assays. After checking the literature, it is recommended to stimulate the cells with some antigen and add...

21 August 2016 417 8 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How the microplastics levels in freshwater and marine water ecosystem be controlled?

Microplastics have been found in land, air, freshwater, effluent from a wastewater treatment plant and even in tap water and bottled water. The small plastic particles (less than 5 mm) pose a...

19 January 2021 8,637 3 View

Why below-ground ecology is still unexplored (majority of it) in our country ?

Below ground ecology or below ground ecological functions and ecosystems roles are very crucial and highly significant for any ecosystem especially for the forest ecosystems. The interaction among...

02 January 2021 6,912 3 View

Anyone an expert in algae in Victoria, Australia?

I found this (very pretty) algae (see picture) in a bore-fed and suburban catchment run-off fed, human made lake (Newport Lakes) in Newport, Victoria, Australia. I'm a vertebrate zoologist with a...

09 November 2020 7,587 3 View

At what time points should pro-inflammatory cytokines levels be tested in a mouse model of Prototheca algae infection?

I'm working on establishing an murine infection model of the Prototheca algae. Not much is known about the dynamics of the infection, nor the induced immune response. I'd like to use the...

19 October 2020 2,908 2 View

What is the possible treatment for Motile Aeromonas Septicemia in fish?

According to Mahon & Lehman (2019), Aeromonas are distributed in a wide range of marine and freshwater environments worldwide. One way of getting infected by Aeromonas is through the ingestion...

18 October 2020 5,564 2 View

Someone knows of a DNA purification protocol from polysaccharides (for brown seaweed extracts)?

Hi, I have this "bad" DNA extract of a Fucus spp. and I want to purifying this because the PCR analysis that I need don't show results. I alredy try to extract twice the DNA both with the Genomic...

06 October 2020 1,500 1 View

Can you send me bigheaded carp occurrence data from European water bodies?

I'm looking for bigheaded carp (silver carp Hypophthalmichthys molitrix and bighead carp H. nobilis) occurrence data from European waters. Faunistic records are mainly published in national/ local...

24 September 2020 4,177 1 View

Which research institutes in India have an algal identification facility?

I am currently pursuing my research in Phycology and planning to conduct a study on microalgae. Is there any place where I can send my samples to be identified? (Preferably in South India) Also,...

17 September 2020 4,440 2 View

How successful use of the DPSIR framework to study the sustainability of wetland ecosystems?

The developed countries have mostly used different approaches, frameworks to achieve the targets of SDGs (Edward, et al., 2007) while restoring the freshwater ecosystems in more productive aspect...

16 September 2020 1,795 7 View