Can I extract mRNA from fixed cells?

Alfonso Blázquez Moreno @Alfonso-Blazquez-Moreno

02 November 2016 1 1K Report

I want to sort my cells using a marker that require intracellular staining and so, fixation and permeabilation. After that staining, and sorting, can I use these cells as source for mRNA extraction?

Erika Barboza Prado Lopes

Ideally you should extract mRNA from live cells, since fixation may influence results, but we all know that some times samples are to valuable to waste. Some people labs do it, so attached you have an article that uses this protocol.

Quantitation of Gene Expression in Formaldehyde-Fixed and Fluorescence-Activated Sorted Cells. Julia N. Russell, Janice E. Clements, Lucio Gama*

http://journals.plos.org/plosone/article/asset?id=10.1371/journal.pone.0073849.PDF

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