Alfonso Blázquez Moreno

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Questions related from Alfonso Blázquez Moreno

Can I extract mRNA from fixed cells?

I want to sort my cells using a marker that require intracellular staining and so, fixation and permeabilation. After that staining, and sorting, can I use these cells as source for mRNA extraction?

02 November 2016 1,361 1 View

Which technique do you usually use for T-cell activation measurement?

I have read about secreted IL-2 measurement as well as Erk, ZAP70 phosphorilation.... Ideally, I would like to measure only the activation generated through CD3Z (CD247) molecule signaling (which...

31 August 2014 2,938 9 View