Alfonso Blázquez Moreno

2 Questions 1 Answers 0 Followers

Questions related from Alfonso Blázquez Moreno

Can I extract mRNA from fixed cells?

I want to sort my cells using a marker that require intracellular staining and so, fixation and permeabilation. After that staining, and sorting, can I use these cells as source for mRNA extraction?

02 November 2016 1,354 1 View

Which technique do you usually use for T-cell activation measurement?

I have read about secreted IL-2 measurement as well as Erk, ZAP70 phosphorilation.... Ideally, I would like to measure only the activation generated through CD3Z (CD247) molecule signaling (which...

31 August 2014 2,936 9 View