Does increasing the number of individuals by copy-paste of the same indvs to perform GWAS affect the results?

I am trying to perform a genome-wide association on mitogenome variants (obtained from GATK-Mutect2) vs. phenotypes by using GAPIT. The number of individuals is seven and GAPIT function does not...

14 December 2020 8,853 3 View

How can I make Isight interact Solidworks with Abaqus?

Hello Everyone, I need to optimize a simple beam natural frequency in Abaqus with Isight. I know in abaqus there is parametric tool but for more complex and further study I want to use Catia or...

25 October 2020 7,007 3 View

Second wave of COVID-19 cases is bigger than the first one in Europe. Do you believe it?

29 September 2020, NewScientist In Europe, the long-predicted second wave of the coronavirus pandemic is now well under way. Several countries are reporting more daily covid-19 cases than they...

11 October 2020 461 5 View

Buffer solution 13 is prepared from a strong base sodium hydroxide and potassium chloride salt ..???

Buffer solution 13 is prepared from a strong base sodium hydroxide and potassium chloride salt .. While the definition of buffer is contain from weak base or weak acid with their salts .. So why...

03 September 2020 7,802 7 View

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12 August 2020 7,970 42 View

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12 August 2020 259 9 View

"Second COVID-19 wave could be twice as big as the first without effective test, trace, isolating strategy." Can you believe it?

Second COVID-19 wave twice as big as the first without effective test, trace, isolating strategy, says new modelling study Reopening schools fully in September without an effective test, trace...

05 August 2020 348 5 View

Only 2.5% of Italians have Covid-19 antibodies, government study shows. Is it good for herd immunity?

4 August, CNN Authorities in Italy have found that only 2.5% of Italians, or 1,482,000 people, have Covid-19 antibodies despite it being one of the countries hardest hit by coronavirus in...

03 August 2020 2,649 20 View

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27 July 2020 4,032 92 View

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Public health officials need to "stick with the science," even in a political atmosphere, Fauci says A pandemic may intensify the divisiveness that already exists in the country, but scientists...

24 July 2020 9,262 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View