Can I design primers to amplify gene of interest from Anoxybacillus using the gene sequences from Bacillus species?

More Khelifa Bouacem's questions See All

What is the most suitable culture medium for Meiothermus ruber?

Dear RG members, If you have an experience with this bacteria, please give me an advice which medium is the best choice for cultivation

11 December 2017 5,774 0 View

Books, reviews, papers evoking microbial enhanced oil recovery ?

Hi All, can someone help me with some books, papers, reviews, thesis talking about microbial enhanced oil recovery? Thanks a lot.

10 November 2017 1,948 1 View

Hi, the casein is difficult to dissolve when solution below pH 6. How do you solve this problem?

09 October 2017 1,828 0 View

Hello, I would like to know what is the best method to screening the keratinolytic and laccase activities ?

Hello, I would like to know what is the best method to screening the keratinolytic and laccase activities from thermophilic aerobic bacteria ?

04 May 2017 2,056 2 View

How to investigate the lipase activity of thermophilic bacteria ?

Could you help me about determination lipase activity in thermophilic bacteria. I will be very grateful for help

03 April 2017 1,082 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Benjamin THOHA Thomas

Dear Khelifa,

Is Anoxybacillus not a memeber of the Bacillus spp? May be you mean to say using a particular specie of Bacillus sequence to design primer for a particular Anoxybacillus. The answer to that is yes especially if you use the conserved region for the primer design. However, using Anoxybacillus sequence for the design is best

Chanuka Kulatunga

Dear Khelifa Bouacem.

Do multiple alignment in the same taxon which includes both Anoxybacillus & bacillus.

Find conserved regions & design primes.

cheers....