Dear all,
While determining the pH stability of a an enzyme. I incubated the enzyme at pH range 3-10 for 72hrs.
I wish to ask, if it is sufficient to describe an enzyme which retains its activity at alkaline pH 10 as "alkaline stable" even though:
1- I did not test stability up to pH 14
and
2- that its optimum activity was pH 5.3 during assay.
Your kind advice and criticism are welcomed.
Dear Ajijolakewu,
yes, your enzyme is an alkaline-stable if it retain activity during at least tens min. However, it is great if it works at alkaline pH.
Thank you Prof. Elisashvili. I was only concerned that I have not considered higher alkaline pH such as 12 or 14.
Thank you once again
I think it can be described like that if it can stay for that period
Dear Ajijolakewu Kamoldeen : If your enzyme retains the activity at pH 10 at least for about 12 hr. then you can name as alkaline enzyme. However, you will have to work out on enzyme half lifeperiod.
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