Interpretation of X-ray reflectivity spectrum?

Dear community, I performed XRR measurements on thin film AuAgSi alloys deposited on Si substrate, please see attached image. For simulation i included 2 nm of SiO2 on top of the substrate and i...

03 February 2021 5,325 2 View

What is the best GIS tool to calculate connectivity score for a point along aquatic network?

Hello, I am looking for a GIS tool (ArcGIS, QGIS, R, etc.) that will calculate a measure of habitat connectivity for nodes along an aquatic (estuarine) network? My dataset is comprised of a...

12 January 2021 6,107 2 View

Can I sequence PCR products from green masterm mix without purification?

I wonder if anybody has an experience of sequencing PCR products where master mix contains green dye for gel loading. What if I will purify them with enzymes instead of gel extraction or...

18 May 2020 5,831 5 View

How to calculate circular dichroism in VASP?

Is there an option to calculate circular dichroism in VASP. If not in VASP then is some free open source software. I need to calculate the spectra for small inorganic nanoclusters (100-200 atoms)

07 May 2019 9,377 3 View

Does anyone detect the 'salt effect' when determining ammona in seawater, highly saline water or brine?

Does anyone detect the 'salt effect' when determining ammona in seawater, highly saline water or brine? How does the time of colouration change when using the indophenol blue reaction in salt...

09 January 2019 1,932 5 View

What types of mouse BM cells can adhere?

07 July 2016 9,616 3 View

Why ROC Curves developed by MATLAB tools are not completely consistent with ROC curves plot from "Principles of underwater sound" (Urick, 1983)?

07 July 2016 474 2 View

Can the antibody alone trigger the FcR-mediated effects in macrophages?

In some models of macrophage activation it can be triggered by the binding of immune complexes to Fc receptors. But in reality free antibodies can also bind FcRs (that's why we use the Fc block in...

18 April 2016 5,292 2 View

I'm involved in CVD graphene preparation and I need a gas diagram (Ar, H2 and CH4) for a CVD equipment. Can anyone help me, please?

Actually I have almost everything for the experiment - chemicals, foil, CVD equipment and so on. And here is the thing - how to adjust the flow of gases?Here you can see a part of a receipt from...

16 March 2016 7,373 3 View

Why are my macrophages positive for both CCR7 and CD206?

I have stained mouse BM with F4/80, CD11b, MHCII, CD206 and CCR7. Cells were Fc-blocked. When gated on F4/80_hi/CD11b_med-hi, I see a population that is CCR7+/CD206+ double positive (see...

31 January 2016 1,192 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View