Oleksandr Zinenko

4 Questions 20 Answers 0 Followers

Questions related from Oleksandr Zinenko

Can I sequence PCR products from green masterm mix without purification?

I wonder if anybody has an experience of sequencing PCR products where master mix contains green dye for gel loading. What if I will purify them with enzymes instead of gel extraction or...

19 May 2020 5,957 5 View

Different nuclear genes or mt-nuclear often results in incongruent genealogy. Did anybody studied why two mt genes result in different tree topology?

Usually when two genes demonstrate different topologies, there is usual explanation that genealogies of these two or more loci were different, they may split in different time, be a subject of...

19 September 2016 3,017 3 View

Can I date hybridization between two populations using information about physical distance between loci?

I have number of SNPs which are mapped on a genome and some proportion of them are linked (100-10000 bp distance). Can I infer time of admixture event? It seems to me that I saw an example of such...

29 January 2016 7,295 2 View

How can one build a tree with not perfect overlapping between sequences?

I have quite not trivial situation. I have a sequence which only slightly overlaps with the rest of alignment (all but 1 sequence in dataset have only few not very informative bp overlap, one...

16 November 2014 8,609 7 View