What is the name of doctor, professor B. Romeis?

In one paper I found a mention of professor B. Romeis, who wrote a book on "Mikroskopische Technik" (1936). I need to mention him in my paper, but I cannot find his full name anywhere. Even in...

13 January 2021 7,402 3 View

After extracting RNA with AllPrep RNA/DNA/miRNA Universal kit, can DNase digestion in solution with heat inactivation damage miRNA/lower yield?

16 September 2020 7,096 3 View

How to improve Desmond MD/metadynamics calculations performance?

Which program parameters is useful to improve calculations performance (ns/day) of MD simulations without simulation quality loss?

28 June 2020 4,840 2 View

Does it make sense to model mass spectrometric fragmentation by DFT?

Hello. I have met a significant number of publications on the study of the laws of mass fragmentation using DFT. Researchers are building huge schemes where they show in which direction the decay...

05 February 2020 6,252 3 View

How to solve problem with momentum overflow in desmond metadynamics simulations?

During metadynamics calculation, Desmond gives error: -- Allowed momentum exceeded on 2 particles. 7187: force = (-3.0580500e+04 4.4492168e+04 -7.2957642e+03) momentum = (-2.9018593e+01...

03 September 2019 8,099 2 View

Is there a formula to calculate minimum required coverage for NGS given a certain variant allele frequency and sequencing error?

I'm looking for a formula that calculates minimum coverage needed to call a variant allele with a certain condifence level, given expected variant allele frequency (VAF) (or percentage of mutants...

05 April 2018 9,336 1 View

Any recommendations as to how many bytes/reads/bp per sample are needed for shotgun/16S metagenome sequencing with genus-deep taxonomic profiling ?

20 March 2018 7,619 4 View

How much Resazurin powder should be added to 10 ml of culture medium to prepare a solution for a mammalian cells viability assay?

I have Resazurin powder from Sigma but I don't know which concentration must have a stock solution or a final solution for viability test. Please, help me.

05 December 2017 7,371 2 View

Stat analysis on the sequence of events

Hi all, I need an advise on the correct statistical approach for a sequence of events. 1. I have a data collected from a group of individuals at baseline (BL) and follow-up (FU). 2. There are...

04 June 2017 8,081 5 View

Does culturing of ASC lead to a 100% pure collection of ASC? Or does it contain other adherent cell types?

Dear all, I am starting an experiment with adipose derived stem cells (ASC), but I would like some help or confirmation for the following issue. Background: I am harvesting murine fat from the...

10 July 2016 3,373 17 View

No title

03 March 2021 5,360 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Has anyone succeeded in capturing uranium atoms in a fullerene structure?

Additional questions are: What are the encapsulating atoms? How difficult was the process and what rates were achieved? What factors most seriously affect the economics?

20 February 2021 3,134 5 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View

What is the relationship that determines the depth of the mechanical cultivator with the age or size of the weed For crops planted in lines?

the great depth of the cultivator may lead to passage under the roots without killing them and may give negative results to break up the soil below the roots, increasing their activity, especially...

03 February 2021 3,198 7 View

Suggestions for using antisense oligonucleotide for brain delivery?

hello I am working on a project that includes gene delivery to the brain. my intentions are encapsulating a gene in a Chitosan NP and IV administration the NP to rats. beside plasmids is there...

29 January 2021 4,455 5 View

Is there a protocol for the insulin encapsulation at physiological pH?

Hello everyone, I am going to start my master thesis on the encapsulation of insulin. In almost all protocols we found an acidic pH is necessary. Unfortunately, pH

27 January 2021 3,692 5 View

Do polymers with different chain lengths have UV-Vis absorption peaks at different wavelengths?

I have a sample of mixtures containing polystyrene microspheres (PS-MS) with 3 different diameter sizes (bought from thermo), they all contain a green fluorescent dye inside the polymer matrix /...

06 January 2021 2,218 5 View

What is this culture medium for?

Here is the composition of a culture medium named BM medium. It doesn't contain carbon source and nitrogen source, while after cultivation something grow on that. Could anyone explain what...

31 December 2020 8,060 5 View

Can anyone explain how to calculate encapsulation efficiency(Plant extract encapsulated alginate nano particles) using total polyphenol content?

Please suggest me if there are any reference materials for this topic. Thank You

30 December 2020 3,799 2 View