How do I dissociate an antibody used for positive selection of cells in order to re-culture the selected cells?

Hi, I am interested in cells that express a specific receptor. I use a fluorophore-conjugated antibody bound to the receptor followed by MACS positive selection to enrich for this...

21 January 2021 2,245 3 View

Is there a standard method for extraction of essential oil of aromatic plant leaves using solar distillation technique?

28 September 2017 4,608 3 View

I am facing problem in dissolving pNPP in 1X diethanolamine. Could any body help me to figure it out?

Hello, We bought pNPP tablets and 5X buffer ( diethanolamine) from Pierce in our laboratory. I have to make 300mM stock solution, (as i can't go to lower concentration for making stock) and it is...

27 August 2015 2,857 1 View

Why MgCl2 is required in all phosphatase assays?

Why MgCl2 is required in all phosphatase assays? Can anybody tell what it does in assay reactions? Thanks

08 April 2015 7,572 2 View

Can any one provide UV-vis Spectra and other related infromation about hydroquinone .

I am trying to make hydroquinone from benzoquinone enzymatically.

05 December 2012 6,712 4 View

A lot of colonies from PCR product

I was doing mutation using PCR, the amount of plasmid that I took was greater than amount given in protocol for digestion of the parental DNA, incubated for more than 4 hours at 37C, then...

23 August 2012 3,353 4 View

Hello friends,

The question is for the people working on fungal proteins. Please tell me on which protein or enzyme from fungus you are working. Thanks vinita

25 January 2012 8,605 5 View

Is the Importance given to dietary DHA just a hype or is it really as beneficial?

DHA is supposedly the omega 3 fatty acid that everyone thinks is really essential and can help in reverting the NCDs if taken at regular times during pre and post natal stages. So is it really true?

17 January 2012 5,126 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View