I performed two experiments. Firstly, a kinase assay in order to assess the effect of various drugs on enzymatic activity of a certain kinase. This experiment was performed with a recombinant kinase enzyme. Secondly, I also measured the effect of the same drugs on the mRNA expression of a gene encoding this kinase in a cell line model. I found the results of these two experiments very similar to each other. Can I perform the regression analysis correlating the data from kinase assay with the relevant data from mRNA expression of a gene? Is it justified even if the data are from different experimental settings? Could you please give examples of papers reporting such correlations?
Thank you!
You can certainly perform the regression analysis if you want to investigate the strength of the correlation between the two effects. If you find one, you must consider whether there is any plausible biological mechanism to explain this correlation. Do not ignore the possibility that it could be non-specific enzyme inhibition, on the one hand, and general cytotoxicity, on the other.
Yes you can correlate the results from the two experiments if you want to determine whether there's a biological relationship between the two effects/activities.
Dear Zain,
Could you please suggest any reference for "integrated cellular pathways approach"?
Thank you,
Thanks to all of you to dispel my doubts with your valuable answers!
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