I want to know two protein interactions via CO-IP, and one of the proteins has several different mutations. I need to transfect all these mutations into HEK293T cells for doing Co-IP every time, and I am concern there would be a big variation between batch to batch experiments, like transfection efficiency, etc. So can I build a stable cell line with all these mutants overexpressed, then I could just spilt cells for each experiment, can this work?

Thanks for reading my question.