Can I autoclave Trsi-HCl, EDTA, Triton-X solution?

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Harout Arabaghian

Hello Chiara,

Regarding Tris and EDTA, we usually sterilize them by autoclaving. As for detergents such as Triton-X, sterilization by filtration (0.22um) is your best choice.

Andrei S. Babenka

Dear colleagues, for me the best way for all components is filtration. Because tris is quite sensitive to heating and sometimes you can receive pH shift. In applications like cloning end oth. mol.biol. manipulations (for ex. transfection) it can affect the results. Of course it is only mine experience.

Chiara Conte

Thanks to all of you for the suggestions!

Rifda Octavia

what sterilization method should i choose for hcl 0,1 N solution?