Want to design a nanocomposite-hydrogel containing growth factors and drug molecule which enhance bone regeneration. But this hydrogel will not contain stem cells.
Can this hydrogel be used as a scaffold to regenerate bone tissue in an affected area?
Are stem cells essential to make scaffold for bone regeneration?
If anyone knows the process, please help me to understand.
Do not worry, stem cells will not do anything. Bone will heal itself and GFs will certainly modulate in either direction. Stem cells alone will make mess. Unless you add stem-cell-differetiated bone cells, that may or may not help.
I injected stem cells to repair osteoarthritis. It damaged the knee much more. But when I transplanted stem cells-differentiated cartilage into knee, that repaired the knee cartilage.
People reporting stem cells everywhere repairing tissues, in my opinion is a bullshit research they are reporting just to publish paper.
Are you trying to regenerate bone or cartilage
Dentists use Aprf.com to good effect when doing dental implants.
I use slow spin PRP (200g for 10 mins as measured at the bottom end of a 10ml tube using a swing basket centrifuge on my own knees for OA and it works well at reducing the inflammatory pain. I doubt that it works by increasing cartilage.
However, by removing all granulocytes the Platelet Factors in PRP appears to be anti-inflammatory
On a technical front the Platelet Factors attract stem cells
The biomechanics of the hydrogel will also make an impact on the degree of regeneration. For example, if the gels are softer ie., stiffness of the gel (compression modulus) is somewhere around 10kPa - 100kPa (kilopascal), bone regeneration will not take place. The gel should have a compression modulus in MPa (mega pascal) range for bone regeneration.
If you are expecting the host cells to infiltrate and populate your gel, it is essential you provide them with appropriate mechanical stimulus along with growth factor/drug. if you provide appropriate stimulus, theoretically osteoblasts will infiltrate the gel, deposit the bone ECM such as calcium, collagen etc and regenerate the bone tissue. (provided that your gel is biocompatible and does not cause immune reaction/capsule formation)
Hope this helps
Thank you for you information. This will be very helpful for me.
Scaffold based on Hydrogel in synthesis can be help to heal and generate defect bone
Modification of Hydrogel by natural and synthetic materials make it promiseing treatment for bone regeneration
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