08 May 2022 3 4K Report

DHU is one of the residues of tRNA commonly used for FL-NHS ester conjugation. H2U is another derivative of U nucleoside. Can that be used for that same type of chemical bonding to label tRNA?

More Aranyadip Gayen's questions See All
What is type-X tRNA of yeast?

Could anybody please let me know in detail what exactly the product is: Ribonucleic acid, transfer Type X, lyophilized powder 9014-25-9 (sigmaaldrich.com)

07 June 2022 7,395 0 View

Why would Anisotropy value come negative?

I have tagged my 12kDa eIF1 protein with Fluorescein-maleimide (Ex: 485nm, Em: 530nm). The interaction with this particular protein with 40S ribosome should show anisotropy value change. 10nM of...

15 September 2020 2,454 2 View

Why housekeeping gene is showing thinner band than targeted knocked down gene with siRNA after PCR?

We transfected MDA MB 231 with SignalSilence® p42 MAPK (Erk2) siRNA, and collected RNA with RNA isolation kit. After cDNA preparation, we set annealing temperature and PCR was done, considering...

02 November 2017 8,110 1 View

Insert having one sticky end and one blunt end. Ligation in pGEMT would be possible maintaining what kind of protocol? Any suggestion ?

insert size=1.3 kb

29 February 2016 9,244 4 View

There are different growth rate in blue-white colony screening of two different genes, using same competent cell for two different genes. Why?

There are different growth rate regarding blue-white colony screening in two plates having two different genes. Though there has been used the same competent cell for two different genes for...

12 February 2016 5,672 4 View

Overlap Extension PCR

I'm getting very less amount of amplification in overlap extension PCR. Fragments I'm using as template are of 516bp and 3.2kb. Please suggest the ratio of mixing of these two fragments. And also...

01 January 1970 8,413 3 View

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