Am I doing the mixed ANOVA right wnen there is many missed values?

01 February 2021 4,695 7 View

How to take into account the high resolution of the x-ray difractometer?

At small angles or in case high collimation, the peaks become anisotropic and with a Lorentz addition. The resolution describes correctly only with adding Lorentz and anisatropy parameters. It...

26 January 2021 5,829 4 View

Meshing a point could and exporting it into an *obj file format?

Anyone, any idea which software will better handle a point cloud to ---> triangular mesh generation with an option to export it into the *obj format? Thanks

01 April 2020 8,459 3 View

How many errors are there in SciFinder and other CAS databases?

14 February 2020 3,016 3 View

I need a rewiew of thermodynamic calculations of my preprint - who can help ?

I have a preprint here: Energy from humid air: reinventing the tornado I need to find the thermodynamics specialist to make a review of the calculations. It is about 600 lines of VBA code in MS...

26 March 2019 8,789 2 View

What metrics are using to compare detection methods?

Hello! In my researches I work with face detection compare methods (on Viola-Jones example). But I still have not found face detection metrics survey (or something like), or object detection...

21 June 2018 4,874 9 View

Dear colleagues, are there any opinions about the modern concept and prospects of development of "Noogenesis"?

05 June 2018 2,310 10 View

Why our universe is nearly flat?

At present, the geometry of the universe is only slightly different from the Euclidean model. This imposes very strong limitations on the curvature of the universe in the early stages of its...

11 April 2018 981 16 View

Are there automatic force field generators/services comparable in quality to OPLS?

What do you think about SwissParam, MolMeccano, GAAMP?

03 September 2017 1,499 4 View

No title

I believe that this species of subfamilies Epilachninae from Africa (Uganda)

08 January 2017 8,811 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View

How to change medium of a protein or peptide mixture?

Hi everyone! I am purifying a peptide from a mixture of proteins. The peptide (3 kDA) is in a solution containing a high concentration of urea and imidazole. I would like to be able to change the...

27 January 2021 4,055 4 View