Alexey Tarabarov

17 Questions 22 Answers 0 Followers

Questions related from Alexey Tarabarov

Could you please suggest methods to compare free protein and immobolized protein binding properties?

I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...

06 August 2024 5,266 0 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

24 July 2024 6,632 6 View

Protein molecular weight appears smaller on SDS-PAGE than expected, anyone knows the reason?

I am working on expression and purification of one cytoplasmic protein with His tag, in E coli Bl21(DE3) host cell. Here is SDS picture. Line 2 and 9 are cell lysate, 3,4,7,8 wash steps and 5,6...

21 March 2024 6,425 5 View

Where can I read some feedbacks about journals?

I would like to know if there is a webiste where people share their experiences about different journals

01 March 2024 8,819 4 View

Are there any methods to separate a nanobody from its antigen?

I am working on nanobody based purification system and in order to reuse it, I have to separate the nanobody from its ligand.

23 December 2023 312 2 View

Who can help analyze the VSM results?

I am constructing Fe3O4 modified nanoparticles, but VSM results seem to differ from previous works.

02 December 2023 1,676 8 View

Do silica and APTMS modified Fe3O4 nanoparticles oxidize faster compare to pure Fe3O4?

I am checking the stabilitty of nanoparticles and found out that the more modifications i make on the surface of MNPs, the faster they oxidize on air. Is it normal?

21 November 2023 9,865 2 View

The best solvent for immobolized nanobody reaction with aflatoxin?

I am doing experiments about nanobody immobilization on nanoparticles and then check the activity of my nanobody with the toxin that my nanobody is supposed to detect. My toxin is in 70:30...

01 September 2023 7,483 4 View

Whats the best technique for monomeric Streptavidin expression and purification ?

I have tried numerous conditions for mSA expression, but still the concentration is very low. Who worked with mSA and can suggest something based on his own experience ?

13 June 2023 5,442 1 View

Can glutaraldehyde react with imidazole buffer?

I performed protein immobilization reaction using GA linker. The results show that the protein was immobolized successfuly, but I was thinking maybe I could improve the results if I remove the...

07 June 2023 9,869 0 View

Can I use Immidazole solution for HPLC?

I am working on toxin detection by one protein and my protein is in immidazole solution. Can i use it for HPLC?

02 June 2023 2,625 6 View

Which GC-MS method(procedure and column type) would you suggest for Aflatoxin B1 determination in solution?

I have zero experience in GC-MS, but have to perform GC-MS to determine the toxin concentration in solution. Any advise would be helpfull. Thanks

20 May 2023 4,064 2 View

Which method would you suggest for toxin concentration determination in solution?

The main problem is that the concentration is very low, usually in pg/ml. Therefore HPLC is not the best method.

18 May 2023 9,298 6 View

Anyone can explain the docking between a protein and a small molecule. How to analyze the results and use it?

I have performed docking analysis between a toxin( small chemical molecule) and it's nanobody. Does anyone have expirence in analyzing the results, i got some reaction sites and possible...

15 April 2023 5,252 3 View

What to do if your FT-IR peaks are overlapped and therefore can’t be distinguished between each other?

I am making the following molecule Fe3O4@SiO2@APTMS- Biotin and after FT-IR it is very hard to prove the presence of Biotin as the main Biotin peak is overlapped by Si-O. Did anyone meet the same...

03 April 2023 9,800 4 View

Why does Biotin have to be linked with NHS before reaction with primary amine?

I have nanoparticles with amino group and I want to cover it with Biotin. I found some papers and all of them say that before the reaction they ahve to modify Biotin with NHS. I wanna know the...

15 March 2023 3,609 1 View

Has anyone tried to immobolize aflatoxin on Fe3O4 nano particles? What kind of mechanism of attachment did you come up with?

Fe3O4@SiO2@TIO2@CPA, 500nm size Any possible help would be appreciated.

27 February 2023 4,527 1 View