The development of genome editing technologies has opened up the possibility of directly targeting and modifying genomic sequences in almost all eukaryotic cells.
It is possible to generate homozygous organisms using gene editing techniques, although the specific timeline for achieving this will depend on a number of factors, including the specific gene editing technology being used, the organism being edited, and the efficiency of the gene editing process.
In general, the process of generating homozygous organisms using gene editing involves introducing a mutation into the genome of an organism, either by using a technology like CRISPR/Cas9 or through the use of chemical mutagenesis. Once the mutation has been introduced, the organisms are allowed to grow and reproduce, and the mutation is passed down to their offspring. Over time, the frequency of the mutation in the population will increase, and it may be possible to generate homozygous individuals that carry two copies of the mutated gene.
It is worth noting that the process of generating homozygous organisms using gene editing can be time-consuming and may require multiple rounds of gene editing and breeding to achieve a high frequency of the mutation in the population. It is possible to achieve it in 2-3 months using certain gene editing technologies and under optimal conditions, but this may not be possible in all cases.