What can explain a low expected heterozygosity rate for T. monococcum wild samples (0.09) ?

I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...

14 February 2021 3,971 1 View

Is there any evidence that using ball milling in intervals, rather than one long run, improves the mixing process?

I am trying to synthesize nanoparticles by mixing the precursors by ball milling. I would like to know if mixing them for one hour straight is less or more efficient than mixing them in intervals...

10 November 2020 8,527 9 View

Can I measure serum and not tissue succinate dehydrogenase?

Dear Colleagues I am interested in evaluating the concentration of SERUM succinate dehydrogenase in a certain type of cancer. I found an ELISA kit for it however, I can barely find any papers...

16 October 2020 547 1 View

How to monitor and mapping crisis event tweets based on my tweets dataset?

how to monitor and mapping crisis event tweets based on my tweets dataset. I need tools for that. I was classified as tweets to damaged and not. then I have extracted locations from textual...

18 August 2020 4,068 2 View

What does it mean by negative values for number of genotypes identified by a primer in detecting the polymorphism ?

So i am recording the polymorphic statistics of iPBS primers and one of the parameters estimated is R (resolving power). From an equation (0,15x + 1,78 = R) i can detect the number of genotypes...

14 February 2019 9,111 1 View

Why Matrix Inverse on Python Gives Different Solution from Excel?

When I compute matrix inverse on Python, it gives me slightly different results from Excel (difference appears after the third or forth number, however it highly affects my work). I know that for...

01 August 2018 2,466 9 View

How can I fluorescently label graphene oxide to be able to see it under confocal microscope?

I have tried to fluorescently label graphene oxide sheets once with Fluorescein sodium salt and another time with Fluorescein isothiocyanate (FITC) by mixing 500 uM of Fluorescein (any of them)...

24 September 2017 1,449 3 View

Can NS2 be used to calculate the memory usage and computation time of a particular agent inside the network node?

I added a security agent to the NS2 core. My aim is to calculate the extra overhead (time and memory allocation) due to the addition of this agent inside the network nodes. Is that possible using...

26 July 2017 7,974 2 View

Can anyone provide me with some papers regarding the nutrient deficiency within the soil in Iran ?

29 January 2017 650 4 View

Is anyone interested in collaborating on this project?

09 July 2015 1,333 15 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View