urinary excretion can give specific data rather than feces, however it is almost difficult to quantify the amount of hormone in the feces.
Thankyou sarker, which are the hormones to be targeted for reproductive status, like adult, pre eostrous, oestrus, post eaostrus, pregnent etc
You can measure the hormone metabolites in Feces. Though, actual hormone concentration is very rare in feces.
Progesterone metabolites can be used to measure the estrous status.
Sanjeeta, Can you help me towards an easy method towards qualitative and if possible quantitative determination of progesterone metabolites in fresh scats obtained from scats
Rubin, I can help you in quantitative determination of progesterone metabolites. There are many papers that will be helpful to you. Kindly go through them.
I would suggest you start with:
Measuring fecal steroids: guidelines for practical application by Rupert Palme. Ann N Y Acad Sci. 2005 Jun;1046:75-80.
http://www.ncbi.nlm.nih.gov/pubmed/16055844
Good luck
There are a number of hormones that can be traced in feces by detecting their metabolites. All the steroid hormones have been well documented in many species. A search for any of the following authors: JL Brown, SL Monfort, L Penfold, L Graham will give you a long list of papers that you can get started on. What species are you looking at?
Steroids have also been studied in birds, but validation for every new species is important because metabolism differs, you can have a look at http://onlinelibrary.wiley.com/doi/10.1196/annals.1343.006/abstract?deniedAccessCustomisedMessage=&userIsAuthenticated=false
Thank you Dear Anneko Moresco, it is very useful and mine is Sloth bear
There is also the Lasley and Kirkpatrick paper (J Zoo Wildlife Med, 1991). Good review article. Also, Ishikawa et al. 2002 (Japanese J Vet Res) did something very similar to what you are interested in pursuing, but with a different species of bear. The feces is dried thoroughly and then the hormones extracted with ether before ELISA. Very standard approach. Good luck.
All the steroids can be assayed in the dungs. See on pub med publications on rhinoceroses from a German research team. Of course the assay needs an extraction before immuno-assay. This extraction is difficult and induced a high variability. The immune assays are ELISA or RIA with tritium.
Sincerely
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