Can exosomes be discriminated based on their surface proteins?

More Stephen W. Holman's questions See All

How do i get an account to upload my published papers?

need to open an account to upload my published papers

01 August 2024 9,255 1 View

How do you determine the Ki of an inhibitor for an enzyme which also undergoes substrate inhibition?

There are three factors involved in the activity, the substrate acting as a substrate, the substrate acting as an inhibitor and another inhibitor for which you want to measure the Ki.

08 July 2024 4,988 1 View

Can anyone help with a sample of Kraft pulp process lime mud (CaCO3)?

Can anyone help with a sample of Kraft pulp process lime mud (CaCO3) I need a sample of 250g to run R&D lab testing. I am based in the UK and I am struggling to get hold of some lime mud...

01 July 2024 9,217 0 View

Can research gate tell me how many times a particular author has been cited?

I'm writing a letter of recommendation for a colleague and I'd like to mention how often her publications are cited by other academics.

28 June 2024 4,995 0 View

How can indigenous knowledge systems assist in mathematics problem solving?

Recent research papers and journals on indigenous knowledge system

21 June 2024 6,358 1 View

L need an assistance on how to carry out a systematic review of causes and preventive measures against maternal mortality in Ogun State, 2018-23?

l need an assistance on how to carry out a systematic review of causes and preventive measures against maternal mortality in Ogun State, Nigeria, 2018-23?

19 May 2024 9,103 1 View

Do imbedded Gaussian basis sets use core potentials (ECP)?

We are using gaussian G-16 to calculate molecular properties. When I specigy in the rout line def2qzvpp ( #P B3LYP/def2qzvpp opt output=pickett) with RU (Ruthenium atom in the atom list) --...

10 May 2024 4,810 5 View

Can Metabolic Disorders cure by medicine,sustitutes,gens,and stemcell excercises etc?

Metabolic disorders occur due to our proper habits of eating process and remineralisation in the mouth. Like food, water, and liquids, etc. Due to mutations in all our food in saliva, the...

22 April 2024 1,959 3 View

Have bears historically preyed on Atlantic salmon (Salmo salar)?

We have searched literature in Norway and North America trying to find evidence that brown or black bears preyed on Atlantic salmon (Salmo salar), despite the fact that these fish spawn in...

17 April 2024 2,262 5 View

What are the explanations as to why apes have been largely confined to tropical & subtropical forests, at least during the Holocene?

As best I have been able to determine from reading literature, chimpanzees, gorillas, bonobos, and orangutans have been largely confined to tropical and subtropical forests, at least during the...

15 April 2024 2,568 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View