Altered Matrix activates the contact Phase of coagulation, then Infusion of prothrombotic Fibrinogen may be risky.
Article Correction of hypothermic and dilutional coagulopathy with c...
According to information from professor Thomas Lindahl in Linkoping, Sweden it can´t. He has written an extensive review, which soon will be published and has several references to it. I know Kenichi Tanaka has a publikation on it. As always it may depend whether it is crystalloid or collloid dilution. I know you are an expert on TG. Also we have recently started to use singe and double centrifugation to study the microparticle affect on the TG and these methodological effects may influence the detectability of dilutional effects on the TG. We could study it together!
Hello Ulf, thank you very much for your interest in a common study on ultrafine TG. Usually, TG is measured by adding TF/PL/Ca2+ to citrated plasma. If you omit TF/PL, your TG gets ultrafine (called recalcified coagulation activity assay = RECA). Only in ultrafine TG you can see low prothrombotic tendencies of plasma supplemented by drugs, H2O, NaCl, or other additives such as microparticles.
I believe the reports of thrombin activation with crystalloids are due to artefact because they are seen only when there is no activator in the system. In such a situation the samples need to be collected in a tube that already contains corn trypsin inhibitor (CTI), to prevent contact activation. My prediction is that if samples are appropriately collected there will be no thrombin activation due to crystalloids in the system already used. We have not done this experiment and I hope someone does it to confirm this and put this otherwise strange observation to rest.
Altered blood matrix activates F12/PK. CTI addition artefactually suppresses this.
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies