Can differential scanning fluorometry be performed to analyse stabilising effect of a hydrophobic substrate on protein using Sypro Orange ?

Mansoor Azeem Siddiqui @Mansoor_Azeem_Siddiqui

08 August 2019 1 8K Report

Sypro Orange binds to hydrophobic residues. Can we use this dye to perform DSF given that our protein stabilisers are hydrophobic molecules themselves.?

Adam B Shapiro

Try the measurement with the substrate but without the protein to see if there are any problems.

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