Mysterious line across SDS gel?

Does anyone have the similar issue? A line across the denature SDS gel shows up after staining with Coomassie blue and there are many lines in every lane if you take a closer look. This gel had 15...

07 January 2021 5,610 4 View

Look for Book?

Can anybody give me the following writer book? Helen Deresky "International Management"

26 December 2020 5,109 5 View

Can anybody help me with an efficient way to de-protect poly(tert-butyl norbornene-2-carboxylate)?

I want to make poly(norbornene 2-carboxylic acid), for the sake of characterization, I decided to start with the protected monomer norbornene-tert-butyl-2-carboxylate for the ring-opening...

03 December 2020 3,852 5 View

Why Schiff bases change colour upon evaporation?

I got bright yellow coloured Schiff base after 6 hour reaction time. So i kept it for slow evaporation. Solvent was methanol. But after complete evaporation my product colour changes from yellow...

17 November 2020 3,824 2 View

What statistical method to use to identify potential confounders in a large set of explanatory variables?

I am working on identifying biomarkers of the outcome of treatment in a disease. I have 20 potential biomarkers and more than 30 potential confounders. Actually, I know that some of those...

11 November 2020 3,794 3 View

Has anyone observed delamination of pyrolysed SU-8 on SiO2 coated Silica substrates when the pyrolysis is performed outside cleanroom?

We have a quartz tube furnace (diameter 80 mm) outside the cleanroom and when tried to pyrolyse the SU-8 on SiO2 coated Si substrates at 900 C in nitrogen atmosphere, the electrodes looks fine....

08 November 2020 4,357 2 View

How can I make my Caki-2 cell line grow?

Hello everyone! I have trouble with a cell line, namely the human kidney carcinoma Caki-2 cell line. Another lab sent us a few criovials of cells, with their growing conditions, but I can't grow...

24 October 2020 2,568 3 View

Look for details for the following reference for citation?

22 October 2020 9,252 4 View

Look for methodology?

Evaluating training design is similar to evaluating trainees reaction?

19 October 2020 4,575 4 View

Look for methodology?

I will do single group pre post test in evaluating training. In that case, what strategy i should follow between experimental or survey research?

03 October 2020 5,326 6 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

Is there any ingredient I an add into formula to slow down gas generation from Vitamin C?

I know Vitamin C is unstable and tends to be oxidized quickly. The problem here is I made a Vitamin C beverage at pH 4-5, and it starts to generate gas in the packaging, especially at high...

13 December 2020 6,055 1 View

Is RabGTPase prenylation reversible or not?

Hi all, I'm working on RAB10, which is a small RabGTPase and is geranylgeranylated at his c-terminal cysteine residues. This is important for membrane insertion. I'm wondering if this...

04 December 2020 4,026 1 View

I need help in peptide oxidation during deprotection group removal?

Hey! I need help on peptide (22 aa) oxidation.This peptide has no methionine, 1 tryptophan & 1 tyrosine. also has 4 cysteine so I used 2 protection groups (trt & acm). For ACM removal, I...

03 December 2020 3,225 2 View

How to reduce (which reducing agent) a disulfide bridge in a protein and how long does it remain reduced?

I want to reduce the disulfide bridge in my 7kDa protein. My protein has only 1 cysteine at the surface and thus makes a homodimer using a disulfide bridge. I want to reduce the disulfide bridge...

24 November 2020 3,211 5 View

What is the best method to check interaction between a drug and a protein ?

Hi, I would like to know the best method to check the interaction between a proteine (cruzipain, cysteine protease) and a drug. It has been purposed to our project to evaluate protein-drug...

30 October 2020 7,209 3 View

Can I alkylate (e.g. iodoacetamide) solvent exposed cysteines of a purified membrane protein??

I have purified a transporter membrane protein and want to perform a CPM assay to assess its melting temperature as apo- and with ligands. The melt scan results in steadily dropping curve while my...

26 September 2020 1,455 4 View

Does a sulfhydryl enzyme necessarily imply a catalytic cysteine?

Could an enzyme be sensitive to sulfhydryl specific inhibitors on account of cysteine(s) away from the catalytic pocket?

13 July 2020 4,165 6 View

Why there is a 11 kDa band showing in SDS-PAGE of my IgG1 digestion with Papain?

Hi guys, I am using the papain digestion system to abtain a Fab. Here is my recent result, I got thin bands around 55, 45; thick bands around 30, 25, which may indicate incomplete digestion, but...

27 June 2020 3,882 9 View

If I have an aqueous solution of ~20-amino acid peptide that contains a single terminal cysteine, will these form disulfide bonds?

I intend to use maleimide chemistry to attach a short peptide with a terminal cysteine to the N-terminal domain of another protein. Do I need to pretreat the short peptide with a reducing agent to...

22 January 2020 227 3 View