I’m trying to quantify cysteine in my sample along with other 11 amino acids using a waters kit method. I am able to quantify all except cysteine.. I seem to get a standard peak but unable to get one in spiked sample
Which are the best ways to analyze the data of PM2.5 & PM10 in the atmosphere collected by gravimetric analysis?
14 April 2024 3,953 3 View
I need to mix two polymers(here the two polymers are taken as the constituent of two paints ) in Ansys Fluent. Hence I need to find out the variation of viscosity of the mixture.
12 April 2024 3,416 0 View
I'm looking for variables like solar irradiation, wind speed, humidity, temperature and precipitation
06 April 2024 1,926 2 View
Currently I am working on the numerical analysis of back to back placed cold formed channel sections with different cfrp wrapping configuration. How can I validate the shear analysis of my...
28 March 2024 3,709 1 View
Im conducting a study on knee osteoarthritis with herbal medication and treatment, was hoping to use WOMAC scale but the license seems to be hard to get. Any leads on how to acquire license for...
27 March 2024 5,174 2 View
Hi everyone, I was wondering if someone has a protocol for using MitoSOX in mitochondrial-enriched extracts. MitoSOX can migrate to the nucleus and generate an inaccurate response about...
24 March 2024 466 0 View
Despite of certain psychiatric conditions that could increase a person's risk of committing a crime, research suggests that patients with mental illness/disorder may be more prone to violence if...
11 March 2024 996 2 View
Would you contribute to a new journal through papers, editorial board, and reviewing? What is your opinion?
04 March 2024 8,502 3 View
Index trends in major states by respective anti-corruption effort by Debroy and Bhandari is till 2012 only. is there any index available till date?
26 February 2024 8,820 0 View
Currently I am working on numerical validation of the paper 'Laboratory and numerical analysis of steel cold formed sigma beams retrofitted by bonded CFRP tapes - extended research' (Laboratory...
25 February 2024 6,380 1 View
Methionine, cysteine, homocysteine, and taurine are the 4 common sulfur-containing amino acids, but only the first 2 are incorporated into proteins. Sulfur belongs to the same group in the...
22 July 2024 4,273 4 View
I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...
02 July 2024 612 6 View
Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...
10 June 2024 4,152 1 View
I have done site-directed mutagenesis (Substitution) to obtain a Cysteine mutation in my gene of interest. Now, I have two domains in my protein, And I want to delete one of the domains to obtain...
05 June 2024 9,430 1 View
As title describes, we're trying to mutate an amino acid of antibody Fc to cysteine, followed by TCEP to further drug conjugation, one of my colleague had raise the concern of purified TCEP...
29 April 2024 3,227 2 View
Hello! I'm trying to simulate AKT1 protein in water, since this protein structure has a disulfide bond between two cysteine residues and some missing residues so I have to model the sequence with...
14 January 2024 5,906 1 View
How to keep L-cysteine from converting to cystine? Even at low pH. Are there any reducing agent may work? better FDA allowed
25 December 2023 4,383 2 View
Hello, I am looking for suggestions for labeling proteins lacking any cysteins and lysines. While, SDS mutation to add a cysteine or lysine are possible options. I am looking for suggestions...
29 November 2023 7,740 1 View
Does anyone know of a method to detect the number of free cysteines on proteins? I would like to assess the binding of quinones to cysteines on an enzyme.
17 November 2023 7,634 2 View
Hi, everybody? I have some peptide which have two cystein. so i want to make cyclized peptide using disulfide bond. but, i have a concern that multimeric peptide by inter-disulfide bond...
02 November 2023 7,422 2 View