Can cysteine and cystine be quantified with waters Accqtag kit method?

22 November 2019 0 822 Report

I’m trying to quantify cysteine in my sample along with other 11 amino acids using a waters kit method. I am able to quantify all except cysteine.. I seem to get a standard peak but unable to get one in spiked sample

Badges
Science topic

More Anjali E Kurian's questions See All

Which are the best ways to analyze the data of PM2.5 & PM10 in the atmosphere collected by gravimetric analysis?

14 April 2024 3,953 3 View

Does anybody know or have conducted simulation of a polymerization process in Ansys Fluent?

I need to mix two polymers(here the two polymers are taken as the constituent of two paints ) in Ansys Fluent. Hence I need to find out the variation of viscosity of the mixture.

12 April 2024 3,416 0 View

Can someone indicates me a database about meteorological variables per country?

I'm looking for variables like solar irradiation, wind speed, humidity, temperature and precipitation

06 April 2024 1,926 2 View

How to validate numerical results of back to back placed cold formed channel sections?

Currently I am working on the numerical analysis of back to back placed cold formed channel sections with different cfrp wrapping configuration. How can I validate the shear analysis of my...

28 March 2024 3,709 1 View

WOMAC scale Indian version license to use?

Im conducting a study on knee osteoarthritis with herbal medication and treatment, was hoping to use WOMAC scale but the license seems to be hard to get. Any leads on how to acquire license for...

27 March 2024 5,174 2 View

Mitosox in mitochondrial extract?

Hi everyone, I was wondering if someone has a protocol for using MitoSOX in mitochondrial-enriched extracts. MitoSOX can migrate to the nucleus and generate an inaccurate response about...

24 March 2024 466 0 View

What is the correlation between schizophrenia and criminal behavior among adults?

Despite of certain psychiatric conditions that could increase a person's risk of committing a crime, research suggests that patients with mental illness/disorder may be more prone to violence if...

11 March 2024 996 2 View

What is a scope of starting a new biotechnology journal from India?

Would you contribute to a new journal through papers, editorial board, and reviewing? What is your opinion?

04 March 2024 8,502 3 View

Do we have anti-corruption index for Indian states?

Index trends in major states by respective anti-corruption effort by Debroy and Bhandari is till 2012 only. is there any index available till date?

26 February 2024 8,820 0 View

How can I solve ABAQUS warnings like negative eigen values, displacement too big for contact and numerical singularity?

Currently I am working on numerical validation of the paper 'Laboratory and numerical analysis of steel cold formed sigma beams retrofitted by bonded CFRP tapes - extended research' (Laboratory...

25 February 2024 6,380 1 View

What plants have high levels of sulfur-containing amino acids such as methionine?

Methionine, cysteine, homocysteine, and taurine are the 4 common sulfur-containing amino acids, but only the first 2 are incorporated into proteins. Sulfur belongs to the same group in the...

22 July 2024 4,273 4 View

Would it be possible to determine how many Cys I have based on Ellman's assay?

I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...

02 July 2024 612 6 View

Media Preparation protocol for Bifidobacteria Agar and Broth?

Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...

10 June 2024 4,152 1 View

Hi, How can I design primers to obtain Deletion mutants by Site Directed Mutagenesis?

I have done site-directed mutagenesis (Substitution) to obtain a Cysteine mutation in my gene of interest. Now, I have two domains in my protein, And I want to delete one of the domains to obtain...

05 June 2024 9,430 1 View

Antibody maleimide conjugation - TCEP method affected by tris concentration in purified buffer?

As title describes, we're trying to mutate an amino acid of antibody Fc to cysteine, followed by TCEP to further drug conjugation, one of my colleague had raise the concern of purified TCEP...

29 April 2024 3,227 2 View

How to do MD simulation of protein with disulfide bond?

Hello! I'm trying to simulate AKT1 protein in water, since this protein structure has a disulfide bond between two cysteine residues and some missing residues so I have to model the sequence with...

14 January 2024 5,906 1 View

How to keep L-cysteine from converting to cystine?

How to keep L-cysteine from converting to cystine? Even at low pH. Are there any reducing agent may work? better FDA allowed

25 December 2023 4,383 2 View

Please suggest dyes for fluorescent labelling of proteins lacking Cysteines and Lysines?

Hello, I am looking for suggestions for labeling proteins lacking any cysteins and lysines. While, SDS mutation to add a cysteine or lysine are possible options. I am looking for suggestions...

29 November 2023 7,740 1 View

Detecting free cysteine on proteins?

Does anyone know of a method to detect the number of free cysteines on proteins? I would like to assess the binding of quinones to cysteines on an enzyme.

17 November 2023 7,634 2 View

How to proceed intra-disulfide bond without inter-disulfide bond formation?

Hi, everybody? I have some peptide which have two cystein. so i want to make cyclized peptide using disulfide bond. but, i have a concern that multimeric peptide by inter-disulfide bond...

02 November 2023 7,422 2 View