We know that cellulase is used to convert cellulose to glucose, but how effective the cellulase and beta glucosidase converts xylan and arabinan? if so, what would be the maximum conversion efficiency for those enzymes?
Most cellulases and no beta glucosidases hydrolyze hemicellulose although a few cellulases also hydrolyze xylan. There are many types of hemicellulose and they each have specific hydrolases.
The hydrolysis of hemicellulose is generally more efficient when using hemicellulases, such as xylanases and beta-xylosidases. However, in my work I used a cellulase "cocktail" from Novozymes (Celluclast 1.5L plus beta-glucosidase Novozyme 188) to hydrolyse a substrate reach in cellulose and xylan and I observed that pratically 100% hydrolyis of cellulose can be achieved, while xylan can also be hydrolysed, but to a small extent.
Here is the reference of my work and other as well that shows this type of behavior:
Bioresource Technology 142 (2013) 198–208
Biotechnology for Biofuels 2 (2009), 14
Hi Ramadoss,
hemicellulases are necessary for hemicellulose hydrolysis, such as xylanases, arabinofuranosidases, feruloyl esterases...
Supporting to the answer of David Wilson. The purified Cellulases and beta-glucosidase are possibly hydrolyzed hemicellulose. It is call " cross activity". IHowever, during the substrate specific experiment, the contamination of small sugar in the substrate used has to be confirmed.
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