Hello,
I am wondering if cell doubling times are universal or do they depend on factors such as plate / flask size? I ask because I've searched some journal articles differ in doubling time of the same cells...
I mean, I would think that they won't change... because doubling time is a part of their nature is it not?
Thanks
Hello Andrew Kim
I mean, I would think that they won't change... because doubling time is a part of their nature is it not?
Cell doubling time does not change. However, it may be altered depending on the environmental factors. For instance, mycoplasma contamination is very common in cell culture. Mycoplasma contaminated cells grow very slowly. Another factor is nutrition. Serum contains growth factors and other important nutrients that allow cells to proliferate. If serum is not provided in required quantity, the cells will not show optimum growth. Also, seeding density affects doubling time.
Other environmental stress inducing factors which include incubator temperature fluctuations, insufficient or inappropriate cell culture substrate as well as inappropriate environmental gas mixture can have an effect on the doubling time.
Best.
Of course, many factors may affect the doubling time of the cells, including the plating density, the type of serum and its concentration, and the type of media you are using. I think even the type of flask o the plate you are using could affect the doubling time.
In some immortalized cell lines also the number of passages.
Valeria
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