How can I prepare disulfiram-copper liposomes using ethanol-based proliposomes method ?

I have a methodology of preparing disulfiram PEGylated liposomes but I am not sure how to attain disulfiram/copper complex in the liposomes.

30 March 2020 4,283 1 View

How can I confirm right colony by PCR?

Hi, I picked up some colonies after electroporation. And I growth those colonies in liquid medium with Erytromycin (200mg/ml). I isolated plasmid after incubation overnight. I did PCR as using...

01 January 2020 9,988 5 View

Significance testing from confidence interval and regression slope?

In a simple regression analysis Seda finds a 95% confidence interval of [-1.4, -0.6] for the unstandardized slope. Is there a statistically significant association between the predictor and the...

07 January 2019 7,050 8 View

SK-MEL 31 cells or B16F10 cells for tyrosinase inhibitor experiments?

Hello Everyone, I have just started to work with animal cell culture studies. I am working on certain novel tyrosinase inhibitors as skin whitening agents. Almost all the literature survey I have...

19 December 2018 8,123 3 View

Why do I see amplification in -RT samples during qPCR?

Hi, During QPCR, I ran non template controls and negative control RT reaction (no RT enzyme using random hexomers and oligodT primers) together with my normal cDNA samples in the same plate. After...

09 July 2018 7,621 4 View

Which steps do you follow after amine synthesis by nitro compounds? and why did the white monomer turn into pink? what can i do not to turn into pink?

02 August 2017 6,211 10 View

Can anyone recommend literature on 'othering'? Is there any socio-pyschological study comparing majority-minority perspectives?

I am specifically asking for socio-psychological studies. I do not like the term "intolerance" since it is politically loaded, but make use of the literature on tolerance, too. I am looking for...

07 September 2015 1,351 6 View

How can I measure the responsivity of a pyroelectric crystal alone?

20 May 2015 5,149 3 View

Which material type is a suitable to design a composite material in ANSYS 14.5?

Thanks in advance for your replies.

28 April 2015 6,558 4 View

Can anyone tell me the lysozyme procedure used for DNA extraction of Lactobacillus sp ?

I need a protocol for lysozyme lysis of Lactobacillus species for DNA extraction from urine and vaginal smear specimens. I have found literatures in which lysozyme lysis is used for Lactobacillus...

11 June 2014 6,886 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View