I made extra Blocking Buffer that I do not want to waste. Can I freeze the blocking buffer to be used later?
How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?
01 August 2024 723 0 View
For an experimental design ( one experiment and one control group), at least or minimum, how many subjects need to be included in the groups in SEM?
08 January 2024 8,210 3 View
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07 November 2023 4,567 2 View
What I want to ask is this: I have a survey and its analysis. I want to build a mathematical model using these analyses. how can I do it
04 September 2023 9,331 3 View
Superoxide Dismutase Assay Method Activity of SOD has been determined in two ways: 1. Inhibition by the enzyme of an O2- dependent reaction. 2. Pulse radiolytic methods (Rigo et al. 1975) See:...
14 August 2023 3,191 1 View
Hello dear researchers In my study, I have 5 independent variables and 1 dependent variable. In multiple regression analysis, not all my IVs significantly predicted my DV. However, most of my IVs...
27 October 2022 8,623 4 View
Hello, I am planning to do gene knockdown using siRNA. Has anyone used siRNA from Synbio Technologies before? If so, what are your thoughts?
19 March 2022 4,655 0 View
I'm using R to run a meta-analysis (using Robust Variance Estimation) of Cohen's d effect sizes. What transformations do I need to do and how do I do them in R? Does anyone have access to online...
13 September 2021 7,239 3 View
Can you help me. I am a student Biology Department. I upload and ran the Native PAGE gel. After 3 hours, even the markers were not visible on the gel. I turned it off and did the painting, but the...
24 June 2021 2,668 2 View
candida albicans biofilm
22 May 2021 9,688 0 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View