I am out of a lab in Wisconsin and recently came across this paper:
Article Preparation of Acute Brain Slices Using an Optimized N-Methy...
Our lab uses chilled sucrose-aCSF during slicing, but I'm wondering if the grass is truly greener. Can anyone attest to which slice prep protocol is better?
And when you tried the NMDG aCSF during slice prep, did you use the sodium spike in solution, as described in the paper above?
Hoping to find answers from people who have tried both. There are many papers which use one or the other, but there isn't a lot of online discussion on which they prefer. Thank you to everyone who responds!
Hello,
in our hands it depends on the age of animal, chilled NMDG-HEPES is superior for older animals (older than 1 month) while sucrose based cutting solution is for younger animals. We did not use sodium spike procedure, and we got good slices for patch-clamping. Personally, I prefer NMDG-HEPES based solution.
Best,
Ljilja
I have not used the sodium spike method but NMDG-aCSF without that is good for the adult age group animals (>P45 days). I was able to get good recordings in voltage clamp conditions.
We regularly use sucrose solutions to cut our slices now. We had tried NMDG briefly at one point in a previous lab, but it was clear that the properties of the neurons we were studying was altered by the NMDG solution and it took most of the day for the effects to wash out/reverse. We haven't had that issue with sucrose so far.
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