Ian Morley

9 Questions 1 Answers 0 Followers

Questions related from Ian Morley

I'm stimulating the shaffer collateral but do not want to generate LTP, what herz am I limited to?

I'm a graduate student at UWM focusing on electrophysiology. I'm trying to perform Shaffer Collateral stimulation of CA1 pyramidal neurons. If I were to have a protocol with 6s of no stim, 2...

19 June 2024 194 0 View

Our -20 Freezer thawed unexpectedly, can freeze-thaw of K-glu internal solution cause issues obtaining Gigaohm seals?

We are an electrophysiology lab out of Milwaukee. Recently, due to ice buildup, a small crack in the door of our -20 freezer resulted in all of the freezers contents reaching room temperature...

07 June 2024 2,086 1 View

What is a good cheap camera from visually guided Whole-Cell Electrophysiology?

Our microscope is an Olympus BX51WI with a C attachment and DIC optics. Fluorescence is not needed. Was wondering what people recommend. I've gotten a few quotes: Regita Electra Pro-about...

20 September 2023 5,638 3 View

Is a 30mOsm difference in internal/external solutions likely to kill neurons while doing Patch-Clamp recorcings?

While testing the osmolarity of our solutions, we noticed our frozen and aliquoted K-internal solutions have an osmolarity ranging from 270-290, while our external aCSF has an osmolarity ranging...

21 August 2023 684 3 View

How often do you need to replace salts? We need help! Do salts ever go bad?

How often do you get new salts? What salts do you get "fresh" most frequently? All help is greatly appreciated. Thank you to everyone who reads or responds, hope you all have a fantastic day. I...

08 August 2023 5,344 4 View

What temperature do you place your brain slices in following dissection and removal when Patching??

Our lab is on the fourth floor of a building without AC. Our protocol involves removing the brain, and immediately slicing in ice cold sucrose. Then we heat the slices back to physiological temp...

22 April 2023 8,869 3 View

How often to make K-internal solution for Patch-Clamping?

Does anyone have insight as to whether it's possible for K-internal to go bad? We are performing whole-cell patch-clamp recordings. For the past few months we have been having issues with cells...

02 February 2023 9,608 4 View

Can anyone verify whether NMDG aCSF is superior to Sucrose aCSF for general cell recovery?

I am out of a lab in Wisconsin and recently came across this paper: Preparation of Acute Brain Slices Using an Optimized N-Methy... Our lab uses chilled sucrose-aCSF during slicing, but I'm...

23 September 2022 8,232 3 View

How can I show Voltage and Current in the same window on Clampex when recording data?

Undergrad learning Patch Clamping here. I was hoping I could imitate an oscilloscope using Clampex. My intuition tells me I have to change the connections on the Digitizer but I am unsure. Anyone...

01 June 2021 9,296 2 View