Ian Morley

5 Questions 1 Answers 0 Followers

Questions related from Ian Morley

How often do you need to replace salts? We need help! Do salts ever go bad?

How often do you get new salts? What salts do you get "fresh" most frequently? All help is greatly appreciated. Thank you to everyone who reads or responds, hope you all have a fantastic day. I...

08 August 2023 5,330 4 View

What temperature do you place your brain slices in following dissection and removal when Patching??

Our lab is on the fourth floor of a building without AC. Our protocol involves removing the brain, and immediately slicing in ice cold sucrose. Then we heat the slices back to physiological temp...

22 April 2023 8,860 3 View

How often to make K-internal solution for Patch-Clamping?

Does anyone have insight as to whether it's possible for K-internal to go bad? We are performing whole-cell patch-clamp recordings. For the past few months we have been having issues with cells...

02 February 2023 9,598 4 View

Can anyone verify whether NMDG aCSF is superior to Sucrose aCSF for general cell recovery?

I am out of a lab in Wisconsin and recently came across this paper: Preparation of Acute Brain Slices Using an Optimized N-Methy... Our lab uses chilled sucrose-aCSF during slicing, but I'm...

23 September 2022 8,220 3 View

How can I show Voltage and Current in the same window on Clampex when recording data?

Undergrad learning Patch Clamping here. I was hoping I could imitate an oscilloscope using Clampex. My intuition tells me I have to change the connections on the Digitizer but I am unsure. Anyone...

01 June 2021 9,267 2 View