Can anyone tell my if there are classic PCR primers for apple scab races (Venturia inequalis)?

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What is the right approach in micro/nanoindentation into the multilayer structure?

In a standard nanoindentation test of thin films, one must be careful about the indentation depth. If the penetration is too low (comparable to roughness), the resulting hardness can be much...

20 September 2022 6,754 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 3,236 2 View

Any suggestion for counting a large amount of small seeds?

I am currently working with S. oleraceus and I would like to know if there is a practical method to accurately counting the seeds other than weighing them or simply counting one by one? Thank you...

02 December 2020 7,199 3 View

How long do the antibacterial coatings work?

Dear all, I am looking for publications dealing with the long-term stability of antibacterial coatings. I need to know if the coating still works even after months or years. The latest term I...

06 October 2020 1,937 5 View

Can anyone advise on OPC culture issues - cell debris and low attachment?

I used to get very clean rat OPC cultures via neurospheres, but for the past year or so (multiple batches overall) I've noticed lower viability in the NPC to OPC phase and I can't figure out where...

25 February 2020 599 0 View

How can i transform an isobologram graph to response surface graph?

I´m researching about synergism between analgesics, and need to do a response surface graph starting of an isobologram. I´m reading about the Loewe model and 3D Isobologram, but I can understand...

17 October 2019 1,253 0 View

Are there any morphological indicators of female reproductive form (other than glair) in crayfish (Genus: Orconectes)?

All the literature I have found on determining whether female crayfish are Form I (reproductive) or Form II (non-reproductive) only mention glair, the presence of eggs, or the actual act of...

02 July 2015 6,052 4 View

Could somebody suggest a protocol for GFP (Green Fluorescence Protein) stain of yeasts?

I want to use GFP to visualize yeast cells in fluorescence microscopy.

03 October 2012 2,764 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View