I want to use GFP to visualize yeast cells in fluorescence microscopy.
My goal is to discover ways to use the new technological discoveries in the arts. The research is part of a study for a PhD thesis
01 April 2024 6,877 0 View
In a standard nanoindentation test of thin films, one must be careful about the indentation depth. If the penetration is too low (comparable to roughness), the resulting hardness can be much...
20 September 2022 6,754 1 View
Can anyone guide me towards existing datasets of dugout and plank boats travel time in open water?
10 June 2022 8,591 0 View
If two classical electromagnetic fields are linearly correlated; can we say the same thing about the fields which describe the same classical fields at quantum scale?
17 March 2022 5,001 0 View
I have been searching for primers (classic PCR) to identify scab races present in my research area. But I have only found scab resistance primers for apple genes of resistance and not primers that...
17 October 2021 8,354 2 View
Hello, Can someone with experience in CT image analysis help me to understand the elements of the pictures. It is a plate thermoformed from waste like ABS and PMMA polymers and fiberglass. I need...
29 July 2021 1,887 6 View
Hi everyone, I'm having some issues with an immunofluorescence staining of the mitochondria in murine cancer cells. I've tried both a MitoTracker and a Tom20 antibody and the signal is always...
04 July 2021 4,513 7 View
I am adapting a scale ( TIntS scale from Pretz, 2014 -23 items) into my native language. After I have done the following steps ( Gudmundsson, E. (2009). Guidelines for translating and adapting...
17 June 2021 953 3 View
I am currently working with S. oleraceus and I would like to know if there is a practical method to accurately counting the seeds other than weighing them or simply counting one by one? Thank you...
02 December 2020 7,199 3 View
Hi everyone, I'd like to ask you all for some advice regarding the feasibility of a certain experiment I'm trying to do. I need to generate a KO cell line in which the expression of a certain...
15 November 2020 2,860 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
average particle size calculation from TEM
04 August 2024 2,921 1 View