I am working on puffers and I have to prove the presence of Tetrodotoxin in them through GC-MS. I processed my fish extract with a standard procedure, and in that I have to evaporate the final volume of the processed extract to dryness and derivatize the residue with derivatizing agent. I evaporated through lyophilizing and direct bunsen flame methods, but the residue I obtained is insoluble in the derivatizing agent called MSTFA. The residue remains separate from MSTFA even after vortexing and boiling. Can anyone suggest me a solution to sort out this issue?
i have not tried this myself, but derivatization and GC/MS analysis of tetrodotoxin is described in this article:
http://cdn.intechopen.com/pdfs/40373/InTech-Instrumental_analysis_of_tetrodotoxin.pdf
For quantification is the only requirement to you, then you prefer other easy techniques like spectrophotometer or HPLC or LCMS
For help in spectrophotometer or HPLC or LCMS contact me
If you wish to do in GCMS, these two will helpful for you
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229236/
http://cdn.intechopen.com/pdfs/40373/InTech-Instrumental_analysis_of_tetrodotoxin.pdf
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