Can anyone tell me the value of surface charge density of DOPG (negatively charged phospholipid) vesicle at neutral pH?

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In Chip-seq, what will be the impact of adding proteinase K before removing the magnetic beads?

I was following this chip-seq protocol https://www.cellsignal.com/products/chip-kits-reagents/plus-sonication-chromatin-ip-kit/56383 what will be the consequence if on the following state "VI....

20 July 2024 5,612 0 View

Researchers, if asked, which 5 Indian Gangster movies would you rate highly or recommend?

Why do I not try one on a Gangster or Mafia movie(s)? This is what I have been thinking for a month or so. Finished the one on ED (Emily Dickinson), and submitted it to a journal.

21 June 2024 2,717 2 View

Would you please share the index of first lines / poem index of the book 100 Selected Poems: Emily Dickinson?

I do have "The Complete Poems of Emily Dickinson."

01 January 2024 5,297 0 View

Will the universe expand forever?

We know that the universe is expanding with acceleration. Can there be any event of such a large scale that can alter the trend of expansion and begin contraction or even stagnant universe?? Lets...

25 December 2023 8,898 3 View

When we add template in a ready to use master mix for PCR amplification, the sample/template get diluted?

I used a established PCR kit to amplify the target DNA. The instructions from kits was given to use (1.5-15ng/ul) concentration of DNA. After DNA extraction procedure i got 23ng/ul;then i adjust...

13 November 2023 4,699 4 View

Any protocol set for fish ethology study with different parameters?

Hi! I am quite a novice in the field of fish ethological research. I am looking for a step-wise guide for parameter choice and data analysis. Especially the movement tracking and behavioural...

08 October 2023 7,282 3 View

Researchers, do you by any chance have any soft copy of the book 𝘓𝘦𝘵𝘵𝘦𝘳𝘴 𝘰𝘧 𝘌𝘮𝘪𝘭𝘺 𝘋𝘪𝘤𝘬𝘪𝘯𝘴𝘰𝘯?

𝙇𝙚𝙩𝙩𝙚𝙧𝙨 𝙤𝙛 𝙀𝙢𝙞𝙡𝙮 𝘿𝙞𝙘𝙠𝙞𝙣𝙨𝙤𝙣 by Emily Dickinson (Author), Mabel Loomis Todd (Editor)

28 September 2023 6,709 1 View

May I get various observational evidences about the existence of diverse (nucleus) acoustic waves in the interiors of compact astrophysical objects?

Dear Colleagues, I would like to request various observational evidences about the existence of diverse (nucleus) acoustic waves in the interiors of compact astrophysical objects, such as white...

17 August 2023 3,363 0 View

Simulation of Transient Analysis of Tunnel FET in Sentaurus?

Can anyone please help in regarding the inverter based on TFET device? I am facing an issue in SDEVICE. I have used the mixed mode code for inverter from manual and used the Tunnel FET physics. I...

25 May 2023 8,583 1 View

Which softwares are better to draw schematic diagrams of beams, plates etc?

I want to know which software is used by authors to draw schematic diagrams of beams, plates etc. for there articles.

22 May 2023 7,631 6 View

Extraction of phaspholipid from soy lecithin?

I am doing an extraction of Phospholipid from soya lecithin but the maximum concentration I am getting is 29.5 % but I need to get it around 50% in HPLC. I am extraction from Acetone and EtOH...

24 July 2024 6,474 4 View

Phospholipids storage conditions?

What is the protocol for storing phospholipids in chloroform solution? Should I divide the contents of a bottle into aliquots to prevent melting each time I take it out for an experiment from...

27 June 2024 2,138 2 View

Lipid composition of the aortic membrane cells (smooth muscles)?

Hello, do you know what the phospholipids of the membrane of rat smooth muscle aortas are composed of? outer membrane-inner membrane

19 June 2024 221 0 View

How Biophysical Composition Index (BCI) can be estimated using ArcGIS Pro? Any youtube video, tutorial, helpbook) please recommend?

Arcgis Pro software preferred.

12 June 2024 3,517 0 View

How to extract phaspholipid from soy lecithin and what maximum concentration can get from soya lecithin?

How do I extract phospholipids from soy lecithin, and what maximum concentration can I get from soy lecithin?

07 June 2024 5,603 0 View

How can I adjust my fabrication method of liposome in order to correctly photographed TEM structures of liposomes?

I preprared curcumin-loaded liposome by Thin Film Hydration Method using lecithin, curcumin and beta-sterol. I stained it with phosphotungstic acid and uranium acetate and took TEM images of each....

30 May 2024 6,863 2 View

Can you help me with a simple method of total extraction of phospholipids from sunflower seed using hexane methanol and water?

I grinded sunflower seeds to fine powder, did Soxhlet extraction using 95% methanol as a solvent overnight. To the resulting solution, i added hexane and water. Put in the separation funnel and...

24 March 2024 6,276 1 View

How can the resistance to movement exerted by fine (non-sandy) sediments be calculated?

I am trying to make a simplified model of the movement of meiofaunal animals in marine sediments. Depending on their mode of movement, meiofauna can be classified as either "interstitial" (i.e....

25 February 2024 7,111 1 View

Best SPE catriges for various analytes?

Do you have any suggestions, which SPE cartiges to use for markers of oxidation stress? I do a LC-MS analysis of quite various group of analytes, including bases (e.g. hydroxyguanosine), acids...

07 January 2024 9,080 7 View

Are there any EEG+ECG+biophysical (immune markers or questionaire) dataset available for Autism Spectrum Disorder (ASD) ?

I am searching for a dataset in ASD that contains EEG+ECG signals and biophysical data of the participants. The biophysical data can be in the form of either blood data (neutrophils, T-cells,...

06 January 2024 8,077 5 View