I grinded sunflower seeds to fine powder, did Soxhlet extraction using 95% methanol as a solvent overnight. To the resulting solution, i added hexane and water. Put in the separation funnel and wait for it to form layers. I got three layers. After evaporating the solvents from each layer through oven drying, the top layer gave oil just like vegetable oil, the middle layer is cloudy yellow like egg yolk, the bottom layer is brown. The yields of the top and the middle layer are the same and the bottom layer has almost a 1\4 of the other yields (v/v). Now i am confused on which layer amongst the two bottom layers contains the phospholipids.
better to go for further extraction with acid-base reaction with organic solvents and after add dil acid the acid aqueous layer my contain.
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