How to add a solar PV generator at bus 14 of IEEE 14 bus system in PSAT?

I am trying to run eigenvalue analysis on a built in IEEE 14 bus test system of PSAT. I need to check how eigenvalues change or shift when a solar PV system is integrated at bus 14. It would be...

28 January 2021 9,294 4 View

Can anyone suggest me possible solution of the following convergence error in the attached file?

I have been trying to derive results by applying a bending load in the parametric cervical spine body part. I tried to define the connection among bodies according to the existing literature....

05 January 2021 2,292 4 View

How to implement truss element with no effect of compression in ansys workbench static structural?

I was trying to add ligaments in cervical spine geometry. There it is mentioned in the existing literature to add the ligaments in the geometry as truss element with no effect of compression....

03 January 2021 2,205 0 View

How can I super impose my 3D structure protein with wild?

Hi I got a 3D protein structures in my research and want to superimpose it with the wild. I am working with SARS-CoV2 and found a deletion that I want to compare with that of Wuhan as a wild. I...

16 December 2020 3,940 5 View

How can I convert my amino acid sequence in PDB formate?

I have some sequences of Spike protein of some Covid 19 samples that have been sequenced in my laboratory using NGS. For analyses of those, I need to convert those sequences into PDB format. I am...

01 December 2020 690 3 View

Why I get this type of band after doing PCR?

After doing a PCR with my specific primer I got multiple bands instead of getting a unique band. I am totally astonished after getting this. Can anyone guessed what happened why I get this band...

26 October 2020 3,714 17 View

What statistical analysis is appropriate?

The data is on vegetation category, disturbances, logging, cutting, distance from the road, elevation, slope, and aspect on a given area from 3 different years. What statistical analysis is...

01 April 2020 1,827 3 View

What is the convenient way to to measure the degree of cooperativity in DNA-DNA or DNA-protein interaction?

Hill equation is used to determine the degree of cooperative behavior in ligand -enzyme interactions. If I would like to utilize this equation in DNA-DNA or protein-DNA interaction, is it...

18 December 2019 5,712 2 View

Why the colony size so small and not cultured in to new LB plate?

New discussion After DNA ligation, I transformed into E. coli and get very small size colony but when I want to make a new LB plate by picking some colony, I did not get any colony culture. I...

04 November 2019 3,275 3 View

Universal sample size calculation through open epi software?

Hi everyone I am doing my research project which is entitled "Knowledge and attitude regarding HPV virus and vaccination against HPV among medical students'. My study population are from 1st year...

26 October 2019 7,128 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

After cutting bands having low molecular weight peptides obtained from SDS-PAGE,is it possible to elute these peptide(s) without digesting in trypsin?

Basically, I want to get the sequence of peptides. I assume, digesting these peptides from each band may give me a different sequence than the original through LC-MS analysis. If digestion doesn't...

30 January 2021 5,482 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View