Can anyone tell me the protocol for pancreatic islets isolation?

03 December 2019 1 4K Report

I am trying to isolate pancreatic islets by infusing 1mg/ml collagenase solution but digestion was not perfect. Any suggestion?

Ekaterine Berishvili

Can you specify, which collagenase are you using and what exactly you mean not perfect. Is your tissue under digested or in contrast overdigested? Depending on this, you will probably need to increase or decrease collagenase concentration or exposition time...

PS: There is huge batch to batch variability in collagenase activity even from same producers.Thats why its better to calculate collagenase activity

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