How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Is BRSV ("bovine respiratory syncytial virus") less susceptible to RNA degradation than SARS-CoV-2 when thawing a sample?

I've noticed that when thawing long-frozen samples (3-4 months at -20 C), most of the SARS-CoV-2 virus does not remain intact, but that the surrogate appears to. Has anyone else noticed this?

09 January 2021 4,168 7 View

Can you centrifuge and re-suspend a cDNA sample?

I am woking with very low concentrations of RNA in an attempt to run a qPCR analysis. Given this, I am going to attempt to synthesize cDNA without diluting my sample, and in two different,...

12 December 2020 3,247 3 View

Is the "Releve" method still standard protocol for plant community sampling and percent cover estimation?

I am not a botanist but need to estimate the average percent vegetation cover of my study area. I have researched this and found articles that use the "releve" method for determining plot size...

22 September 2020 5,676 6 View

How to accelerate Langevin dynamics simulations for bead-spring polymer chain models?

I'm running simulations for a bead-spring polymer networks using LAMMPS. I need my simulations to reach biologically relevant timescales of at least a few milliseconds. However each simulation...

14 September 2020 2,954 1 View

Convex Optimization for Deep Learning?

Is there such a thing as convex optimization for large deep learning networks? Is there a way to guarantee convergence? Has anyone looked into this?

06 August 2020 5,384 4 View

What happens in post-secondary education when instructors don't use traditional grades?

I am looking for empirical studies that examine the use of non-traditional grading in higher education. This means NOT using A-F grades during a course (even if, at the conclusion of the course,...

09 June 2020 1,519 6 View

What is the role of journals' reviewers? Should they be absolute judges?

- Ask questions where they are not sure/convinced about a statement/conclusion VS reject a statement/conclusion where they 'think' it is not correct? - Provide suggestions to improve a...

21 May 2020 2,624 7 View

Looking for immunologist or virologist to help with literature-based study for COVID treatment. Can you help?

On the side, I doing some research (literature-based) on a particular treatment that may help for COVID-19 symptoms but I need someone with a stronger medical knowledge base with expertise in...

29 April 2020 8,098 11 View

Female Soccer - Small sided games - Does training intensity reduce when elite players train with non elite ? or senior players with lower ages groups?

Hi , Im wondering does anyone know of any papers detailing the effects to training intensity of small sided games when the group is of mixed performance levels i.e( professional& semi...

18 December 2019 6,495 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

How can I find the thermophysical properties of high temperature microencapsulated phase change materials (MPCM)?

Hi everyone. I am looking for a high temperature MPCM. More specifically, I need the thermo physical properties of a microencapsulated phase change material with the melting temperature between...

14 February 2021 3,606 2 View

Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View