Can anyone tell me please how to elute protein from precipitated beads?

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Valerie Amsellem

I use leammli buffer and boil for 10 min that release protein from beads.

Alexander Streng

See my answer to the following question:

https://www.researchgate.net/post/How_can_I_elute_protein_from_immunoprecipitated_beads?_tpcectx=profile_answers

Sreenivasulu Kilari

You can elute your protein with 200mM Glycine buffer, pH 2.0

Muhammed Soylemez

Thank you all guys for your answers...