i have isolated these fungi from a plant source and i want to know colony characteristic of these fungi.
Dear Vipin,
What you have provided are the macrocultures of fungi. Unless you provide the details of microculture of the same, it would only be a wild guess to identify even the genus.
Microculture preparation observed under low, medium and high magnification would even help to idetify at both genus and species level. Ofcourse sometimes you also have to take help of macroculture characteristics in addition to microculture properties.
Fungi are identified by their colony characters on certain media. Colony characters on obverse and reverse , texture, colour, pigment, diffused pigment colour. One can not identify without these. Both colonies are velvety, furrowed and mottled.There is diffused pigment in both plates. Colour will change in pictures.
Identification of moulds is based almost entirely on the structures bearing spores or conides and on the spores themselves. So it is important to observe the culture at the microscope. The process of identification usually involves keys, specialized flow-charts leading to the name of the organism. The most common means of identifying moulds is by the use of a dichotomous key, a device presenting a series of alternatives for consideration. All the information can be found on specialized books. It is difficult to recommend one or even a few books on identification Ainsworth, Sparrow, and Sussman (1973) and Arx (1981) offer keys to most of the groups of fungi. If the mould under examination appears not to be an ascomycete, basidiomycete, or zygomycete, try starting with Barron (1968), Barnett and Hunter (1987), or Carmichael et al. (1980). The latest mould identification book is The Genera of Hyphomycetes by Seifert, Morgan-Jones, Gams and Kendrick (2011). PCR techniques are also useful for molds identification.
Dear Vipin Nagda
I agree with Lucia Bonadonna. For identification of molds we need some cultural characteristics (such as morphology of colony, colony color and pigments production) and growth rates of them on various media (based on keys and literature papers). By the way, microscopic characters will be useful for identification of these groups of fungi. Molecular techniques are also useful for correct identification of molds.
Regards: Hamid
You should study the spore and mycelia morphology under microscope.. Molecular identification will be more helpful..
The colonies of these two plates are similar to Penicillium sp. However, you better make slide and check for the microscopic characters under the microscope to confirm about this then.
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