Thermophiles possess reverse DNA gyrase, which catalyzes the positive supercoiling of closed circular DNA (by contrast, nonhyperthermophiles contain DNA gyrase, an enzyme that supercoils DNA in a negative twisted fashion), Hope this helps.

Dear sir..........C.Lauzon

I had gone through 3-4 research papers i got much information but its concluded that even though reverse gyrase adds positive coils its nothing to do with stability of hyperthermophiles......i wants to know more about this can u please tell me currently who is working on this topic.

Why don't you try PUBMED. May be it will help you to know who is working on such a topic and where.

Dear sir..........Jai Ghosh

Thank u for your suggestion.I got 3-4 papers in PUBMED only but,i dint get current status who is working and where .

True, yet while some have found conflicting results, others still see its role in DNA stability. For example, check out Jamrose et al. 2013 J Biolog. Chem. Taken from their abstract: " Our results suggest for the first time that a reverse gyrase shares not only structural but also functional features with evolutionary conserved helicase-topoisomerase complexes involved in genome stability".

Dear sir................C.Lauzon

Ya ur true ............i will chick that paper.............thank u sir.