Can anyone suggest why PAS staining is not working for a band?

29 May 2014 0 2K Report

It stains well some other proteins but not my protein of interest which is secreted by a recombinant strain of Pichia (well known to over glycosylate secretory proteins), recombinant protein is predicted to have 4 potential O-glycosylation site using ExPasy software. Anyone can provide insight or an alternative method?

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