I preserved my specimen into 70% ethanol for a long time. Following dehydration, I placed them to the CPD but they were pulverised in CPD. Can anyone provide me with a cpd protocol for plant material?
Soil: Mohr-Coulomb
27 November 2023 6,581 0 View
Valid questionnaire to assess prevalence of Multimorbidity
19 May 2023 4,274 0 View
What are the laws, regulations, zoning guidelines, etc., for preventing negative impacts of noise coming from infrastructure, especially renewable energy? Some countries have more lax...
20 January 2023 2,724 2 View
Which ethical metrics is expected from an academic who is the owner or the creator of the instrument? The measurement tool will be applied to adults as an online form and a snowball sample will...
01 February 2021 9,528 3 View
I'm looking for any kind of legislation, zoning, best practices, etc. either required or recommended by the state, the EU/other federation or local government.
17 January 2020 5,149 2 View
I'm looking for methodologies, if there are any, to assess the impact of different peace-building efforts. Is there a way to assess whether a peace-building effort is successful other than if it...
24 July 2017 7,882 1 View
I'm looking for articles or books on the notion of private vs. public in landscape architecture, architecture and design. Anyone know anything about this? General insight is also good.
24 May 2017 2,916 2 View
For those of you who perform in depth interviews as part of a qualitative or mixed-methods study. How many interviews are considered as enough in your field (and by international peer reviewed...
27 April 2017 7,994 24 View
I could not find any materials that describe the difference between a formal and informal situational analysis on the internet. However, the requirement for my project clearly states that I need...
02 January 2017 6,993 6 View
Hi! I made DIG labeled RNA probe for ISH through in vitro transcription. I am wondering why I saw 3 band on agarose gel. It is because polymerase amplified other form of DNA (supercoil,circular...
16 December 2015 3,762 5 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
31 July 2024 8,309 0 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color
28 July 2024 286 0 View
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24 July 2024 3,087 3 View
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,592 3 View
I want to study the differences between two groups: the treatment group and the comparison group. The total population consists of 60000 women in the treatment group, distributed across different...
21 July 2024 669 3 View
can any one guid me about tractical method for preservation of microorganism same as bacteria, cyanobacteria and algea, in sampling from environment to Laboratory: 1- Sample collection 2-...
17 July 2024 3,687 11 View
Can we preserve the isolated protoplasts for future experiments? Is it possible to preserve them via cryopreservation? If yes how long can we preserve them?
16 July 2024 9,576 1 View