Which ethical metrics is expected from an academic who is the owner or the creator of the measuring instrument?

Which ethical metrics is expected from an academic who is the owner or the creator of the instrument? The measurement tool will be applied to adults as an online form and a snowball sample will...

01 February 2021 9,188 3 View

QPCR considerations in analyzing samples from the field?

I am attempting to plan a qPCR experiment in which I evaluate the expression of multiple genes in various brain regions of fetuses, as a function of location in utero (intrauterine position,...

31 October 2020 5,788 1 View

What purity grade of PEG-300 and NMP should be used to dissolve drug for in vivo use?

Hi. I need to dissolve a drug to be given to mice by oral gavage. Based on a paper I found, the drug should be dissolved in 10% (vol/vol) NMP/40% (vol/vol) PEG-300/50% (vol/vol) water. Looking...

16 August 2020 8,685 2 View

Question for psychtoolbox users. Divided the screen?

*Question for psychtoolbox users* I using one screen. The function "flip" flips all screen. Can i divided the screen to two areas of "flip"? I have two stimuli with different time display flipping...

10 August 2020 4,828 3 View

Which mouse strain is best suited for HCT116 xenograft experiments?

I would like to perform a xenograft experiment with HCT116 cells on immune compromised mice. I was wondering which mouse strain would be best suited for such an experiment?

05 August 2020 9,437 3 View

How to conduct moderation model in a mixed design?

I have a mixed model in which I have: - within independent variable with three levels (emotion regulation strategies) - between independent variable, continuous (fear of height questionnaire...

24 May 2018 4,107 2 View

Can any one give me a protocol for beta gal ONPG assay for AML12 transfected cells?

I have a protocol which we used a few years ago with 3T3 cells. With the AML12 cells I don't get any color. Any suggestions? Thank you.

30 October 2017 1,749 1 View

How to increase the yield of islets isolated from RAT?

01 February 2016 3,138 17 View

Advantages and inconveniences using adjusted area under the curve (aAUC) to study tumor growth ?

Hi ! I would like to know if there is inconvenients in using aAUC to stydy tumor growth in xenograft mice ? I also would like to know if bootstrap method is good to estimate a confident interval,...

05 January 2015 1,787 3 View

Protocol for fatty acid extraction from mammalian tissue - can anyone help?

I need to extract and identify the fatty acid composition (with GC-MSMS) of several tissues harvested from mice.

22 October 2013 1,414 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View