Can anyone suggest the reason for pink-colored colonies of Burkholderia pseudomallei in MacConkey agar media?

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Phil Geis

This is Ashdown's article describing the namesake medium - note comment Absorption of neutral red was found to be suitable for differentiating this organism from other bacteria..." Article An Improved Screening Technique for Isolation of Pseudomonas...

Jameel M. Abduljalil

Even colonies of B. cepacia appears pink on MacConkey agar due to extended incubation period. The pH indicator of the medium responses to acidity from any other source, not only carbohydrate fermentation reactions.

Gilberto Antonio Eskildsen

It is correct, this bacterium is lactose negative and its colonies are observed pinkish rugose colonies on MacConkey's agar. Its color is possibly due to the an intrinsic pigment production and may vary due to phenotypic switching.

You can read the paper: Biological Relevance of Colony Morphology and Phenotypic Switching by Burkholderia pseudomallei at the journal of bacteriology

Arittra Bhattacharjee

Thank you for your responses. These answers will really help me.

Robert John Wolff

It was nice to see this discussion both because there was a clear question, but even more importantly the answers were knowledgeable with helpful information. When possible references should always be given, and several of the answers would have been more helpful if there was a citation that could be checked out.