Other than plant tissue culture, any other propagation technique to grow Chlorophytum borivilianum. The plant species is endemic to North India. Is it possible to cultivate it in the South?
I guess the natural methods for the species to propagate are by seed and its tuberous roots. The germination rate of the seeds seems to be rather low, up to 25%, but you might be able to improve on this by experimenting. The other method would be by planting (pieces of) the root. Also here you would need to experiment how long these pieces should be. An advantage of propagation by roots is that they are genetically identical, but a disadvantage is that the roots are the parts that you want to use medicinally.
This species grows in tropical conditions, does tolerate low temparatures but no frost, and needs shade.
It has tuberious roots so you can cut explants from the tubers and propagate much more than other explants taken from this plant. Think it like potato regenerations you can find numerous protocols for that.
Also i agree with Dr. Schmelzer, left the plant to cold degrees as like fridge's +4 degree part before you use.
C.borvbillianum is a plant with tuberous root. But it doesn't have any node or bud like potato. If you plant root tuber alone don't get any plantlet. Best practice for propagation in all native Chlorophytum (C.tuberosum, C.orchidariun, C.arundinaceum, C.laxum etc. ) is spliting the disc (place of attachment of the tubers to the stem portion) with at least one tuber with it and planting the same. You may refer a thesis by Dr. Naresh Kumar Bisen from FRI Universityfor more details.